human chondrosarcoma sw 1353 Search Results


human chondrosarcoma cells sw 1353  (LGC Promochem)
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LGC Promochem human chondrosarcoma cells sw 1353
Northern blot analysis of mRNA from various connective tissue cell lines. (a) A radiolabelled PH-20 cDNA probe was hybridized to a northern blot containing 25 μg/lane of total RNA from (1) testis (positive control); (2) liver (negative control); (3) synoviocytes; (4) chondrocytes; (5) chondrocytes stimulated with 5 ng/ml of IL-1; (6) chondrocytes stimulated with 10 ng/ml of IL-1; (7) fibroblasts; (8) a <t>chondrosarcoma</t> cell line. (b) For loading control, the same blot was stripped and hybridized with a β-actin probe. (c) Column box–whisker plot showing the relative increase of the PH-20 2.4-kilobase transcript in chondrocytes stimulated with IL-1 at two concentrations ( n = 5; * P = 0.0004 by paired t -test).
Human Chondrosarcoma Cells Sw 1353, supplied by LGC Promochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Northern blot analysis of mRNA from various connective tissue cell lines. (a) A radiolabelled PH-20 cDNA probe was hybridized to a northern blot containing 25 μg/lane of total RNA from (1) testis (positive control); (2) liver (negative control); (3) synoviocytes; (4) chondrocytes; (5) chondrocytes stimulated with 5 ng/ml of IL-1; (6) chondrocytes stimulated with 10 ng/ml of IL-1; (7) fibroblasts; (8) a chondrosarcoma cell line. (b) For loading control, the same blot was stripped and hybridized with a β-actin probe. (c) Column box–whisker plot showing the relative increase of the PH-20 2.4-kilobase transcript in chondrocytes stimulated with IL-1 at two concentrations ( n = 5; * P = 0.0004 by paired t -test).

Journal: Arthritis Research & Therapy

Article Title: Chondrocytes, synoviocytes and dermal fibroblasts all express PH-20, a hyaluronidase active at neutral pH

doi: 10.1186/ar1730

Figure Lengend Snippet: Northern blot analysis of mRNA from various connective tissue cell lines. (a) A radiolabelled PH-20 cDNA probe was hybridized to a northern blot containing 25 μg/lane of total RNA from (1) testis (positive control); (2) liver (negative control); (3) synoviocytes; (4) chondrocytes; (5) chondrocytes stimulated with 5 ng/ml of IL-1; (6) chondrocytes stimulated with 10 ng/ml of IL-1; (7) fibroblasts; (8) a chondrosarcoma cell line. (b) For loading control, the same blot was stripped and hybridized with a β-actin probe. (c) Column box–whisker plot showing the relative increase of the PH-20 2.4-kilobase transcript in chondrocytes stimulated with IL-1 at two concentrations ( n = 5; * P = 0.0004 by paired t -test).

Article Snippet: Human chondrosarcoma cells (SW 1353) were purchased from the American Tissue Culture Collection (LGC Promochem, Teddington, UK).

Techniques: Northern Blot, Positive Control, Negative Control, Control, Whisker Assay